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University Email Address:
slei@medicine.nodak.edu
Office Phone:
701-777-4745
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Keywords:
Neurotransmitters, Neuropeptides, Synaptic transmission
and plasticity, Ion channels, Learning and memory,
Synaptogenesis, Neurodegenerative disease, Epilepsy,
Anxiety, Hippocampus, Entorhinal Cortex, Interneuron,
Patch-clamp, Electrophysiology
Education/Training:
- Postdoctoral training, University of Toronto,
National Institutes of Health
- Ph. D. Molecular and cellular pharmacology,
University of Alberta
- MD. Medicine, Sun Yat-sen University
- Msc. Biochemical Pharmacology, Sun Yat-sen University
Research
Activity:
We use a variety of techniques including electrophysiology,
immunocytochemisty, imaging, tissue culture, molecular
biology and in vivo physiology and animal models
to study the functional changes of the central nervous
system in physiological and pathological conditions.
Our current research interests include:
- Modulation of synaptic transmission and plasticity
in the hippocampus and entorhinal cortex. Using
infrared video microscopy and patch-clamp recording
techniques, we are able to record the synaptic
activity or ion channel currents from visually
identified neurons in slices. The synaptic activities
and ion channel functions are modulated by numerous
modulators including neurotransmitters and neuropeptides.
We are exploring the underlying cellular and molecular
mechanisms using molecular biology, pharmacology
and transgenic animal models.
- The functional changes of neurons induced by
the neurotransmitters and neuromodulators are
likely to be responsible for a variety of physiological
functions such as learning and memory or clinical
disorders including epilepsy, anxiety and neurodegenerative
diseases. We are using in vivo disease models
to study the roles of these neuromodulators in
these clinical disorders. Our research would likely
to provide clues at the molecular and cellular
levels to treat neurological diseases.
Selected Research
Papers:
- Deng
PY, Poudel SKS, Rojanathammanee L, Porter JE, Lei
S. Serotonin Inhibits Neuronal Excitability
by Activating Two-pore Domain K+ channels
in the Entorhinal Cortex. Molecular Pharmacology
72:208-218, 2007
- Deng
PY, LEI S. Long-term depression
in identified stellate neurons of juvenile rat entorhinal
cortex. Journal of Neurophysiology 97:727-737,
2007
-
Deng PY, Porter JE, Shin HS, LEI S.
Thyrotropin-releasing hormone increases GABA release
in rat hippocampus. Journal of Physiology
577:497-511, 2006
-
Deng PY, LEI S. Bidirectional Modulation
of GABAergic Transmission By Cholecystokinin In
Hippocampal Dentate Gyrus Granule Cells of Juvenile
Rats. Journal of Physiology 572:425-442,
2006
- LEI
S, McBain CJ. Two loci of expression for
long-term depression at hippocampal mossy fiber-interneuron
synapses. Journal of Neuroscience 24:2112-2121,
2004
-
- LEI,
S. and McBain, C. J. GABAB Receptor modulation
of Excitatory and Inhibitory Synaptic Transmission
onto rat CA3 Hippocampal Interneurons. Journal
of Physiology 546:439-453, 2003
-
Arundine M, Chopra GK, Wrong A, LEI S,
Aarts MM, MacDonald JF, Tymianski M.
Enhanced Vulnerability to NMDA Toxicity in Sublethal
Traumatic Neuronal
Injury In-Vitro. Journal of Neurotrauma
20:1377-1395, 2003
- LEI,
S. and McBain, C. J. Distinct NMDA receptors
provide differential modes of transmission at mossy
fiber-interneuron synapses. Neuron 33:921-933,
2002
- LEI,
S.,
Czerwinska, E., Czerwinski, W., Walsh, M. P. and
MacDonald, J. F. Regulation of NMDA receptor activity
by F-actin and myosin-light chain kinase. Journal
of Neuroscience 21:8464-8472, 2001
- Atzori,
M., LEI, S., Evans, D. I. P., Kanold, P.
O., Phillips-Tansey, E., McIntyre, O. and McBain,
C. J. Differential synaptic processing separates
stationary from transient inputs to the auditory
cortex. Nature Neuroscience 4:1230-1237,
2001
- LEI,
S.,
Dryden, W. F. and Smith, P. A. Nerve growth factor
regulates sodium but not potassium channel currents
in sympathetic B-neurons of adult bullfrogs. Journal
of Neurophysiology 86: 641-650, 2001
- LEI,
S., MacDonald, J. F. Gadolinium reduces AMPA
receptor desensitization and deactivation in hippocampal
neurons. Journal of Neurophysiology 86: 173-182,
2001
- LEI,
S., B. A. Orser, G. R. L. Thatcher, J. N. Reynolds,
and J. F. MacDonald. Positive allosteric modulators
of AMPA receptors reduce proton-Induced receptor
desensitization in rat hippocampal neurons. Journal
of Neurophysiology 85: 2030-2038, 2001
- LEI,
S,
Jackson MF, Jia Z, Roder J, Bai D, Orser BA, MacDonald
JF. Cyclic GMP-dependent feedback inhibition of
AMPA receptors is independent of PKG. Nature
Neuroscience 3 : 559-565, 2000
- LEI,
S, Lu WY, Xiong ZG, Orser BA, Valenzuela CF,
MacDonald JF. Platelet-derived Growth Factor Receptor-induced
Feed-forward Inhibition of Excitatory Transmission
between Hippocampal Pyramidal Neurons. Journal
of Biological Chemistry 274 : 30617-30623, 1999
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Lu W-Y, Xiong Z-G, LEI S., Orser B.A., Dudek
E., Browning M, D., MacDonald J. F. G-protein-coupled
receptors act via protein kinase C and Src to regulate
NMDA receptors. Nature Neuroscience 2: 331-338,
1999
- LEI,
S.,
Dryden, W. F. and Smith, P. A. Involvement of Ras/MAP
kinase in the regulation of Ca2+ channels
in adult bullfrog sympathetic neurons by nerve growth
factor. Journal of Neurophysiology 80: 1352-1361,
1998
- LEI,
S., Dryden, W. F. and Smith, P. A. Regulation
of N- and L-type Ca2+-channels in adult
frog sympathetic ganglion B-cells by nerve growth
factor in vitro and in vivo. Journal of Neurophysiology
78:3359-3370, 1997
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